How does an amperometric sensor work?
The principle of amperometric sensor is based on measuring current generated by enzymatic or bioaffinity reaction at the electrode surface, at a constant working potential with respect to the reference electrode.
What is amperometric electrode?
Definition. Amperometry is one of a family of electrochemical methods in which the potential applied to a sensing electrode is controlled instrumentally and the current occurring as a consequence of oxidation/reduction at the electrode surface is recorded as the analytical signal.
What is amperometric titration used for?
Amperometric titration is the standard method for comparison of accuracy in determining residual chlorine in drinking water. FAC is determined by titration with a standard solution of As(III) (in the form of phenylarsine oxide, C6H5AsO) at a pH between 6.5 and 7.5.
How is potential selected in amperometric titration?
In Amperometric titrations, the potential applied between the indicator electrode (dropping mercury electrode) and the appropriate depolarizing reference electrode (saturated calomel electrode) is kept constant and current through the electrolytic cell is then measured on the addition of each increment of titrating …
What are 1st generation 2nd generation and 3rd generation amperometric biosensors?
Biosensors are classified based on the electroactivity of the bio-receptor substrate or product (first generation), the use of redox mediators (second generation), or direct electron transfer between the redox-active biomolecule and the electrode surface (third-generation) [62] .
What are potentiometric sensors used for?
1.4 Potentiometric sensors. Potentiometric sensors mainly determine the analyte concentration by measuring the variation of potential difference between working and reference electrodes at different analyte concentrations. Ion-selective electrodes belong to such sensor. The typical example is pH meter.
Which electrode is used in amperometric titration?
What are the three generations of biosensors?
There are three so-called ‘generations’ of biosensors; First generation biosensors where the normal product of the reaction diffuses to the transducer and causes the electrical response, second generation biosensors which involve specific ‘mediators’ between the reaction and the transducer in order to generate improved …