What is BrdU cell proliferation assay?
BrdU Cell Proliferation Assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells. It is sensitive, rapid, and easy to perform.
What is BrdU cell cycle?
BrdU staining of cells for cell cycle analysis and apoptosis. BrdU is an analog of thymidine readily incorporated into DNA during DNA synthesis. It provides an accurate method of monitoring proliferation and apoptosis. The Mouse Anti-BrdU antibody, clone Bu20a, is suitable for flow cytometry.
What cells does BrdU stain for?
Bromodeoxyuridine (BrdU) is one such common nucleotide analog that is classically used to track label‐retaining cells (LRCs) after a prolonged “washout” period that dilutes the label within the more rapidly cycling transient‐amplifying (TA) cells.
How do you do a BrdU assay?
Prepare a 10 mM stock solution of BrdU (ab142567) by dissolving 3 mg of BrdU in 1 mL water. Dilute the 10 mM BrdU stock solution in cell culture medium to make a 10 µM BrdU labeling solution. Filter the 10 µM BrdU labeling solution through a 0.2 µm filter under sterile conditions.
What is BrdU antibody?
BrdU is a thymidine analogue and when offered to proliferating cells it is incroporated into reduplicating cells. The antibody is specific for DNA in which BrdU has been incorporated. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides.
What is a BrdU assay?
Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes.
How does BrdU staining work?
Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. Once incorporated, these nucleoside analogs serve as cell cycle and proliferation markers that can be detected using labeled probes to identify cells that are actively proliferating.
How to prepare BrdU cell proliferation assay kit 6813?
Specific for product: BrdU Cell Proliferation Assay Kit #6813. Prepare 1X Wash Buffer by diluting 20X Wash Buffer (included in each BrdU ELISA Kit) in purified water. Prepare 1X detection antibody solution by diluting BrdU Detection Antibody 1:100 with Detection Antibody Diluent (green).
How is BrdU used as an indicator of cell proliferation?
The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells.
How to prepare 10x BrdU solution for plate cell culture?
Prepare 10X BrdU solution by diluting BrdU 1:100 with cell culture medium. Plate cells in 96-well plate and incubate with respective test substance. Typical seed cell number is 2500–100000 cells/well depending on cell growth rate. Typical incubation time is 1–72 hr. Add prepared 10X BrdU solution to plate wells, for a final 1X concentration.
How is MCF 10A cells treated with BrdU to increase cell proliferation?
Serum was added to the plate at various concentrations and cells were incubated for 24 hr. Finally, 10 μM BrdU was added to the plate and cells were incubated for 4 hr. Figure 2. Treatment of MCF 10A cells with Human Epidermal Growth Factor (hEGF) #8916 increases cell proliferation as detected by BrdU Cell Proliferation Assay Kit #6813.