What is sequence specific oligonucleotide?
The polymerase chain reaction (PCR) is an in vitro technology of amplifying defined DNA sequences to a high copy quantity from an extremely small number of genomic or cDNA template.
What is biotinylated probe?
Biotinylated probes for in situ hybridization (ISH) are now widely used to detect RNAs and viral genomes at the light and electron microscopic levels. Many protocols for ISH with biotinylated probes are now available.
What are oligonucleotide gene probes?
Oligonucleotide probes are short stretches of single-stranded DNA or RNA used to detect the presence of complementary nucleic acid sequences (target sequences) by hybridization.
What is a probe in sequencing?
A probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome. The probe is placed into contact with the sample under conditions that allow the probe sequence to hybridize with its complementary sequence.
What is SSP PCR?
Sequence-specific amplification (SSP) is simply a form of polymerase chain reaction (PCR) which involves designing one or both primers so that they will or will not allow amplification (the 3′-mismatch principle). Its origins are probably legion, i.e. many people probably thought of it at the same time.
How are oligonucleotide probes produced?
Oligonucleotide probes are often prepared with covalent attachment of a reporter molecule (eg, a fluorescent dye) or affinity labels that allow them to be attached to solid supports. Probes used in homogeneous (real-time) PCR are usually oligonucleotides with a fluorescent label.
How are oligonucleotide probes designed?
The probes are designed to hybridize with the polymorphic sequences in the target HLA locus. Under appropriate hybridization and wash conditions, these probes will bind only to their complementary sequence in the amplified DNA and are able to distinguish single nucleotide differences.
Why are biotinylated oligonucleotide probes useful for the analysis of pituitary endocrine function?
It is emphasized that biotinylated oligonucleotide probes are useful for the analysis of pituitary endocrine function because they are applicable to the three hybridization methods, namely, Northern blot hybridization and ISH at the light and electron microscope levels.
Are biotinylated probes used for light microscope ish?
The same biotinylated probes were used not for light microscope ISH but also for the electron microscopical demonstration of rat growth hormone and prolactin mRNAs on the polysomes of the rough endoplasmic reticula.
Can Northern blot hybridization detect rat growth hormone and prolactin mRNAs?
Using biotinylated probes, gene transcripts of rat growth hormone and prolactin were detected by Northern blot hybridization. The same biotinylated probes were used not for light microscope ISH but also for the electron microscopical demonstration of rat growth hormone and prolactin mRNAs on the polysomes of the rough endoplasmic reticula.