What does the latex test for Staphylococcus aureus detect?
White latex particles are coated with human fibrinogen for detection of clumping factor and specific IgG for detection of protein A….Staphaurex™ Latex Agglutination Test.
| Control Sets | Positive |
|---|---|
| Detectable Analytes | Clumping Factor and Protein A |
| Type | Staphylococcus aureus Test Kit |
What is latex latex agglutination?
Latex agglutination is observed when a sample containing the specific antigen (or antibody) is mixed with an antibody (or antigen) which is coated on the surface of latex particles. The reaction between a particulate antigen and an antibody results in visible clumping called agglutination.
Is Staph aureus latex positive?
Positive latex tests occurred in 98.3% of coagulase-positive strains, whereas 99.6% of coagulase-negative strains gave negative latex tests. It is concluded that in most instances, the latex slide agglutination test is a reliable method for identifying S. aureus in the clinical laboratory.
Why is latex agglutination testing commonly used?
Latex agglutination testing, also called latex fixation, is a diagnostic study that is widely used as a laboratory method to identify certain antibodies and antigens. The test uses a variety of body fluids including blood, urine, saliva and cerebrospinal fluid, which are dependable on what type of sample is needed.
Does S aureus agglutinate?
Staphylococcus aureus is a prominent bacterial pathogen that is known to agglutinate in the presence of human plasma to form stable clumps.
When is latex agglutination test appropriate?
The test can be also used for rapid detection of bacterial antigens in body fluids to recognise and treat diseases such as pneumococcal infection, Haemophilus influenza type b (which is recognised as a factor in number serious diseases such as blood poisoning, infection of the lungs or infection of the lining …
What bacteria is detected using the latex agglutination test?
A modified latex agglutination (LA) test performed on glass slides was evaluated for the detection of Streptococcus pneumoniae (Pn), Hemophilus influenzae type b (Hib), and K1 antigens in serum, cerebrospinal fluid (CSF), nasopharyngeal secretions, and other body fluids in patients suspected of having infection.
What is the principle of the latex agglutination test for RF?
PRINCIPLES OFTHE PROCEDURE The principle of the test is an immunologic reaction between the Rheumatoid Factor (RF), a macromolecular molecule globulin found in serum and the corresponding lgG coated onto finely dispersed polystyrene latex particles.
What is coagulase positive Staphylococcus?
Staphylococcus aureus and other coagulase-positive staphylococci (CoPS) are opportunistic pathogens associated with a large spectrum of diseases that range from skin and mucosal infections to life-threatening septicemias in humans and animals.
What are two types of staphylococcal coagulase?
Coagulase is an enzyme-like protein and causes plasma to clot by converting fibrinogen to fibrin. Staphylococcus aureus produces two forms of coagulase: bound and free. Bound coagulase (clumping factor) is bound to the bacterial cell wall and reacts directly with fibrinogen.