What is Pen Strep used for?
Penicillin Streptomycin solution, also known as Pen Strep, is the most commonly used antibiotic solution for the culture of mammalian cells. Penicillin Streptomycin solution is used to maintain sterile conditions during cell culture, and they show few adverse effects on the cultured cells.
Is Pen Strep an antibiotic?
Pen/strep is the dream team of antibiotics for cell culture purposes. Where penicillin interferes with the turnover of bacterial walls, streptomycin binds 30S subunits of bacterial ribosomes—the combination of both results in the inhibition of protein synthesis and ultimately the death of susceptible bacteria.
What is 100X Pen Strep?
Penicillin/Streptomycin (Pen/Strep) is an antibiotic mixture, which can be added to cell culture media. This solution is effective against contaminations with gram-positive and gram-negative bacteria.
How do you dilute a 100X strep Pen?
The accurate way would be to dilute 5 mL of Antibiotic-Antimycotic (100X) into 495 mL of medium. Please note that the volume of medium in a 500 mL medium bottle is not exactly 500 mL. It could range from 500.5 – 510.5 mL.
Can you give dogs Pen and Strep?
Dosage and Administration Cattle, sheep, goats, horses, camelids and swine: 1 mL/25 kg of b.w. by intramuscular route; dogs and cats: 1 mL/10 kg of b.w. Apply through intramuscular route (subcutaneous route could be used in dogs).
How much Pen and Strep Do sheep need?
The recommended daily dose is 1ml per 25kg bodyweight for cattle, horses, pigs and sheep. The dose should be given once daily by deep intramuscular injection for up to three consecutive days.
How long does Pen-Strep last in media?
Finally, I know media (DMEM) by itself is generally stable for 12 months from manufacture when stored refrigerated, and sera is generally stable for 5 years when stored at -20°C or colder.
Is Pen and strep long acting?
Meat: 31 days. Cattle, Milk: 60 hours. Milk for human consumption must not be taken during treatment. Meat: 23 days.
How Much is a strep Pen?
In general, 50-100 units of Pen-Strep per milliliter of media is used to avoid contamination in cell culture. Thus, the retail product is generally 100 times more concentrated. It is recommended for use in cell culture applications at a concentration of 10 ml per liter.
How do you store a strep Pen?
While Pen-Strep is stable at 37C for up to 72h, you will save a lot of time if you have smaller volumes to thaw. Store at -5 to -20C. White/pale precipitate is common after thawing.
How much Pen-Strep do you add?
Between 0.5 and 1 mL of Penicillin-Streptomycin solution are added to 100 mL of cell culture media for a final concentration of 50 to 100 I.U./mL penicillin and 50 to 100 µg/mL streptomycin.
What does 100X concentration mean?
The “X” factor simply indicates that the solution is in a concentrated form that must. usually be diluted to a “1X” concentration for use. For example, a 5X concentrated solution must. be diluted 5-fold, while a 100X concentrated solution must be diluted 100-fold. The dilutions.