What is the embryonic origin of the pancreas?
The pancreas and other gastrointestinal organs are derived from the endoderm. The pancreas is formed in the uterus from the fusion of two separate pancreatic ducts, the dorsal and ventral. The dorsal pancreatic bud first appears at approximately embryonic day 9.5 (E9.
How do zebrafish induce diabetes?
Combined High Cholesterol Diet (HCD) and High Glucose (HG) Environment Model of Hyperglycemia for Adult Zebrafish. In another study, Wang et al. (2020) revealed that exposure to 2% glucose solution and consuming 10% cholesterol diet for 19 days induces hyperglycemic state in adult zebrafish.
Where is the pancreas found in the fish?
Between the stomach and the intestine, ducts enter the digestive tube from the liver and pancreas. The liver is a large, clearly defined organ. The pancreas may be embedded in it, diffused through it, or broken into small parts spread along some of the intestine.
Why are zebrafish good model organisms?
As zebrafish eggs are fertilised and develop outside the mother’s body it is an ideal model organism for studying early development. Zebrafish have a similar genetic structure to humans. They share 70 per cent of genes with us. 84 per cent of genes known to be associated with human disease have a zebrafish counterpart.
What week does pancreas develop?
The pancreas first appears at approximately 5 weeks of gestation as two outpouchings of the endodermal lining of the duodenum just distal to the forming stomach (Figure 5). The outpouchings are the ventral and dorsal pancreas. The dorsal pancreas grows more rapidly than the ventral pancreas.
How does hyperglycemia occur?
Hyperglycemia, or high blood glucose, occurs when there is too much sugar in the blood. This happens when your body has too little insulin (the hormone that transports glucose into the blood), or if your body can’t use insulin properly. The condition is most often linked with diabetes.
What is fish pancreas?
In adult fish the main pancreas is located on the right side attached to the lateral aspect of the intestine by the pancreatic duct. Typically, one large islet and 3-6 smaller islets occupy the main pancreas.
What is the function of pancreas?
During digestion, your pancreas makes pancreatic juices called enzymes. These enzymes break down sugars, fats, and starches. Your pancreas also helps your digestive system by making hormones. These are chemical messengers that travel through your blood.
How do zebrafish lay eggs?
Zebrafish lay eggs which are fertilized by the male after they are laid. Your fish is probably full of eggs and waiting for a male (usually skinnier and slightly yellow on the bottom side) to mate with. She won’t lay the eggs without a male.
Can zebrafish regenerate body parts?
Adult zebrafish are able to regenerate different organs, including all fins (32), the spinal cord (33), the retina (34), the heart (35), the telencephalon (36), and the kidney (37). Interestingly, the mechanisms that control regeneration seem to be organ-specific.
Which pancreatic buds appear first?
largerdorsal pancreatic bud
Most of the pancreas is derived from the largerdorsal pancreatic bud, which appears first and develops at a slight distance cranial to the ventral bud. The smallerventral pancreatic bud develops near the entry of the bile duct into the duodenum and grows between the layers of the ventral mesentery.
How does zebrafish contribute to the study of pancreas?
Zebrafish has penetrated this field very rapidly offering a new model of investigation; by joining functional genomics, genetics and in vivo whole mount visualization, Danio rerio has allowed large scale and fine multidimensional analysis of gene functions during pancreas formation and differentiation. Animals Cell Differentiation
How can apoptosis be assessed in zebrafish embryos?
Several methods have been described for assessing apoptosis in zebrafish embryos. Acridine orange, which stains apoptotic cells in living embryos, has been used in young embryos to show broad spatial distribution of apoptosis ( Jensen, Gitlin, & Carayannopoulos, 2006 ).
What animal models can be used to study pancreas formation and differentiation?
Until 1996, the main animal models in which pancreas formation and differentiation could be studied were mouse and, for some instances related to early development, chicken and Xenopus.
What is the function of the vertebrate pancreas?
The vertebrate pancreas is composed of acinar tissue that produces digestive enzymes, a ductal system for transporting those enzymes, and the endocrine islets which produce hormones critical for organism glucose homeostasis. Recent studies have highlighted similarities between zebrafish and mammals …