What is a pGEX vector?
pGEX-2TK is designed to allow the detection of expressed proteins by directly labeling the tagged products in vitro. This vector contains the recognition sequence for the catalytic subunit of cAMP-dependent protein kinase obtained from heart muscle.
How does tac promoter work?
The tac promoter is used to control and increase the expression levels of a target gene and is used in the over-expression of recombinant proteins. The tac promoter is named after the two promoters which comprise its sequence: the ‘trp’ and the ‘lac’ promoters.
What is the sequence of lac promoter?
The lactose (lac) operon promoter is positively regulated by the catabolite gene activator-cyclic AMP complex (CAP) that binds to the DNA located 61.5 bp upstream of the transcription start site. Between the CAP binding site and the core promoter sequence is a 13-bp sequence (from -38 to -50 [the -45 region]).
What is pribnow sequence?
The Pribnow box (also known as the Pribnow-Schaller box) is a sequence of TATAAT of six nucleotides (thymine, adenine, thymine, etc.) that is an essential part of a promoter site on DNA for transcription to occur in bacteria.
What is the correct sequence of proteins found in the lac operon?
In the lac operon, these sequences are called P (promoter), O (operator), and CBS (CAP-binding site). Collectively, sequence elements such as these are called cis-elements because they must be located on the same piece of DNA as the genes they regulate.
How long is the lacZ gene?
LacZ gene size
| Value | 3075 base pairs |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Benno Muller-hill, 1996, The Lac Operon: A Short History Of A Genetic Paradigm, Publisher: Walter de Gruyter. Berlin. New York. Part 3.2 pp.134 |
| Entered by | Uri M |
| ID | 102070 |
What is 35 sequence?
The -35 position, simply titled the -35 element, typically consists of the sequence TTGACA and this element controls the rate of transcription. Bacterial cells contain sigma factors which assist the RNA polymerase in binding to the promoter region. Each sigma factor recognizes different core promoter sequences.