What is BG-11 medium used for?
BG11 Broth is a universal medium for the cultivation and maintenance of blue green algae (cyanobacteria).
Why is BG-11 medium for cyanobacteria?
Gibco® BG-11 Media is optimized for the growth and maintenance of select Cyanobacteria including Synechococcus elongates. The formulation comes ready-to-use at 1X to let you avoid laborious media preparation steps.
How to prepare BG-11?
Combine the following solutions and adjust pH to 7,5 (Use 1.0M HCl or NaOH). Stock Per liter of medium Stock 1 10 ml Stock 2 10 ml Stock 3 10 ml Na2CO3 0,02 g Stock 4 1,0 ml Page 2 NaNO3 1.5 g (For 100x BG-11 solution don’t add NaNO3) For solid media: add 1.5% agar to the liquid BG11 media.
Who discovered BG-11 medium?
BG-11 Medium (modified by J. Acreman) | Canadian Phycological Culture Centre | University of Waterloo.
How do you isolate cyanobacteria from water?
To isolate cyanobacteria from lakes and ponds in which cyanobacterial growth is not visible with the eye (or even after examination with a portable microscope) it is advisable to take larger samples: 250- to 500-ml sterile screw-cap centrifuge pots, filled almost completely with sampling water, are convenient …
How do I make BBM media?
- Fill a clean 5 l Schott bottle with 4625 ml MilliQ water.
- Add 50 ml of stock solutions 1,2,3,4,5,6 (stored in the fridge in the main lab)
- Add 5 ml of stock solutions 7,8,9 (stored in the fridge in the main lab)
- Add 10 ml of stock solutions 10.1, 10.2, 10.4, 10.5 (stored in the cabinet)
How do you make Zarrouk’s medium?
The medium (Rajasekaran et al. 2016 ) used in culture experiments consisted of (per liter) 16.8 g NaHCO 3 , 0.625 g K 2 HPO 4 , 2.5 g NaNO 3 , 1 g K 2 SO 4 , 1 g NaCl, 0.1 g MgSO 4 , 0.04 g CaCl 4 ·2H 2 O, 0.01 g FeSO 4 · 7H 2 O, 0.08 g Na 2 EDTA·2H 2 O, and 1.0 mL of trace element solution.
Which medium used for isolation of cyanobacteria?
A method employing nutrient saturated glass fiber filters allowed the isolation of the same numbers of cyanobacteria from freshwater as were obtained with medium solidified with agar, while providing a 2- to 15-fold reduction in the number of accompanying heterotrophic bacteria.
What are the stock solutions for basic bg11 medium?
B) For basic BG11 medium combine the following stock solutions: Stock Solution Per Liter of medium Stock 1 10 ml Stock 2 10 ml Stock 3 10 ml Na2CO3 0.02g Stock 5
How do you make bg11-n medium?
BG-11(-N) Medium. BG-11(-N) Medium Recipe. Directions. Liquid media: To approximately 900 mL of dH 2O, add the first eight (8) components in the order specified while stirring continuously. Bring the total volume to 1 L with dH 2O. Cover and autoclave medium. Allow to cool then store at refrigerator temperature.
What is the purpose of storage temperature BG 11?
storage temp. BG-11 is an universal medium for the cultivation and maintenance of blue green algae (cyanobacteria), such as Synechocystis 6803; Nostoc sp. PCC 9104 and Nostoc sp. PCC 9025. It is used for studies of the photosystems, as well as to identify the primary targets of the photoprocess.
How to prepare bg-11o from noble agar?
Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 ml/125 ml flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11o don’t add NaNO3.
