What is pHrodo dye?
pHrodo dyes can be used to measure intracellular pH in either the cytosol or in cellular compartments. The minimal dye fluorescence at neutral pH also eliminates the need for wash steps and quencher dyes because any non-internalized dye is essentially nonfluorescent in the culture medium.
Can pHrodo be fixed?
pHrodo is relatively non-fluorescent until it enters the acidic phagosome, at which point its fluorescence increases. If you fix the sample, the pHrodo will only reflect the pH of the buffer the cells are in, and not the pH of the phagosome. For this reason, we do not recommend fixing samples.
What is pHrodo?
pHrodo Red AM Intracellular pH Indicator is a novel fluorogenic probe used to measure intracellular pH in live cells. pHrodo Red is weakly fluorescent at neutral pH but increasingly fluorescent as the pH drops.
What is pHrodo Red?
pHrodo Red is a fluorescent compound with an excitation peak at 560 nm and an emission peak at 587 nm. It can be excited using a 561 nm laser paired with a 582/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSAria™ Fusion.
What is pHrodo green?
pHrodo Green AM Intracellular pH Indicator is a novel fluorogenic probe used to measure intracellular pH in live cells. pHrodo Green is weakly fluorescent at neutral pH but increasingly fluorescent as the pH drops.
What does a Phagolysosome do?
In biology, a phagolysosome, or endolysosome, is a cytoplasmic body formed by the fusion of a phagosome with a lysosome in a process that occurs during phagocytosis. Formation of phagolysosomes is essential for the intracellular destruction of microorganisms and pathogens.
How does a phagolysosome differ from a phagosome?
The key difference between phagolysosome and phagosome is that phagolysosome is a cytoplasmic body formed by the fusion of a phagosome with a lysosome. Meanwhile, phagosome is a vesicle formed around the particles engulfed by a phagocytic cell during phagocytosis.
What is phrodo® E?
The fluorescence of the novel pHrodo® dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes. Use the ready-made pHrodo® E.
What is phrodo used for in microbiology?
The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes. Use the ready-made pHrodo® E.coli BioParticles® conjugates in imaging or flow applications, or pHrodo® SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
How do I use phrodo® products?
Use the ready-made pHrodo® E.coli BioParticles® conjugates in imaging or flow applications, or pHrodo® SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice. For Research Use Only. Not for use in diagnostic procedures. Store in freezer (-5 to -30°C) and protect from light.
What’s new in phrodo® dye conjugates?
New pH-sensitive pHrodo® dye conjugates give faster and more accurate results than any other phagocytosis assay. pHrodo® dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes. Get faster staining and more accurate results—without the need for wash steps or quencher dye.