What is Surface plasmon resonance analysis?
Introduction. Surface plasmon resonance (SPR) binding analysis methodology is used to study molecular interactions (1, 2). SPR is an optical technique for detecting the interaction of two different molecules in which one is mobile and one is fixed on a thin gold film (1).
What is the use of surface plasmon resonance?
SPR is a powerful label-free method widely used to study binding between two macromolecules [12–15]. Typically, one of the two interacting partners is immobilized on a sensor chip surface, and the other is flowed through a microfluidic system in contact with the chip surface.
What is resonant biosensor?
The Resonant Mirror biosensor is a new design of optical sensor, aimed at combining the improved sensitivity of waveguide sensors with the simplicity of fabrication and ease of use associated with surface plasmon sensors.
What is surface plasmon resonance PDF?
Surface plasmon resonance (SPR) is a phenomenon occuring at metal surfaces (typically gold and silver) when an incident light beam strikes the surface at a particular angle.
What is surface plasmon resonance in drug discovery?
Abstract. Surface plasmon resonance (SPR) spectroscopy is a rapidly developing technique for the study of ligand binding interactions with membrane proteins, which are the major molecular targets for validated drugs and for current and foreseeable drug discovery.
What affects surface plasmon resonance?
Surface plasmon resonance (SPR) is the manifestation of a resonance effect due to the interaction of conduction electrons of metal nanoparticles with incident photons. The interaction relies on the size and shape of the metal nanoparticles and on the nature and composition of the dispersion medium.
Which biosensor works on basis of resonance frequencies?
Piezoelectric biosensors are of two types: the quartz crystal microbalance and the surface acoustic wave device. They are based on the measurement of changes in resonance frequency of a piezoelectric crystal due to mass changes on the crystal structure.
