What is the difference between M CSF and GM-CSF?
M-CSF exhibits a mostly homeostatic expression pattern, whereas GM-CSF is a product of cells activated during inflammatory or pathologic conditions. Accordingly, M-CSF regulates the numbers of various tissue macrophage and monocyte populations without altering their “activation” status.
What is a cytokine GM-CSF?
GM-CSF is a monomeric glycoprotein that functions as a cytokine—it is a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes.
What are THP-1 monocytes?
THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology research, and toxicology research.
What does GM-CSF stand for?
A substance that helps make more white blood cells, especially granulocytes, macrophages, and cells that become platelets. It is a cytokine that is a type of hematopoietic (blood-forming) agent. Also called granulocyte-macrophage colony-stimulating factor and sargramostim.
What does THP-1 stand for?
THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and immunohistochemistry.
What does THP-1 cells stand for?
THP-1 designates a spontaneously immortalized monocyte-like cell line, derived from the peripheral blood of a childhood case of acute monocytic leukemia (M5 subtype) (3).
How long to incubate THP-1 cells before differentiation?
Hello all, my current experiments involve using THP-1 cells that have been differentiated into immature dendritic cells. According to the literature, this is done by incubation of the cells for, from what I can tell, a minimum of 5 days in 100ng/mL GM-CSF and IL-4, replacing the media every 2 days.
How is THP-1 cell suspension added to the insert?
2 ml of the THP-1 cell suspension is added into the inserts (2 × 105cells/insert). Once settled THP-1 cells are treated immediately with 10 ng/ml of 12-O-tetradecanoylphorbol-l3-acetate (PMA) for 24 h (1 μl of stock solution into 4 ml total volume) as described in Figure 1Day 1. Open in a separate window Figure 1
How do you count THP-1 cells?
THP-1 cells are counted using a haemocytometer a cell suspension with a concentration of 1 × 105cells/ml is made up. One 6 well-plate is then prepared by adding 2 ml of RPMI/FCS media per 6 well. The transwell inserts are then placed into the 6 well containing the 2 ml medium.
How much PMA do you put in THP1 cells?
2 ml of the THP-1 cell suspension is added into the inserts (2 × 105cells/insert). Once settled THP-1 cells are treated immediately with 10 ng/ml of 12-O-tetradecanoylphorbol-l3-acetate (PMA) for 24 h (1 μl of stock solution into 4 ml total volume) as described in Figure 1Day 1.