How do you differentiate U937 cells?
Abstract. Human promonocytic cell line U937 cells can be induced to differentiate into macrophages by treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). TPA treatment induced the expression of the monocytic differentiation markers CD11b and CD36, with concomitant morphological changes.
Why are U937 cells used?
U937 cells were isolated from a histiocytic lymphoma of a 37-year-old male patient in 1974. Because they are one of just a few cell lines available that express many monocytic characteristics, they are often used to study the behavior and differentiation of monocytes.
What type of cells are U937?
U937 is a pro-monocytic, human myeloid leukaemia cell line and was isolated from the histiocytic lymphoma of a 37 year old male (Sundstrom and Nilsson 1976). This cell line exhibits many characteristics of monocytes and is easy to use.
How do monocytes differentiate into macrophages or dendritic cells?
Abstract. Monocytes can give rise to either antigen presenting dendritic cells (DCs) or scavenging macrophages. This differentiation is initiated when monocytes cross the endothelium.
Is U937 an AML cell line?
The U937 cell line was originally established from the pleural effusion of a patient with histiocytic lymphoma and is usually considered to be an AML cell line (10, 11).
Are U937 cells adherent?
Culture of U937 Cells U937 cells were differentiated by culture at a starting cell concentration of 1 × 106 cells/ml in medium containing 100 nM phorbol 12-myristate 13-acetate (PMA) for 48 hr. The cells were then adherent and somewhat flattened.
How do monocytes become dendritic cells?
Monocytes differentiate into dendritic cells under inflammatory conditions in peripheral tissues. However, as early as the 1990s, Sallusto and Lanzavecchia15 and Romani et al. demonstrated that human monocytes differentiate into DCs in vitro by culturing with GM-CSF and IL-4.
Why do monocytes differentiate into macrophages?
Macrophage differentiation from monocytes occurs in the tissue in concomitance with the acquisition of a functional phenotype that depends on microenvironmental signals, thereby accounting for the many and apparently opposed macrophage functions.
What are A549 cells?
A549 cells are lung carcinoma epithelial cells that constitute a cell line. A549 is one of the commonly used cell lines for a wide range of research applications.
What are L929 cells?
L929/A cells can be used in the development of novel anti-cancer treatments. Resistance can be circumvented by modulating agents such as verapamil and quinine. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue. Also Known As: Species: Mouse.
Do monocytes differentiate into large phagocytic cells?
Monocytes differentiate into large phagocytic cells called macrophages.
Are dendritic cells different from macrophages?
Macrophages refer to a type of white blood cells that surround and kill microorganisms, remove dead cells, and stimulate the activity of other immune system cells. Dendritic cells refer to a special type of immune cells that boost immune responses by showing antigens on its surface to other cells of the immune system.
Do U937 cells express CD1a and CD1b?
We also found that U937 cells do not express measurable levels of CD1a, CD1b or CD1c following conventional differentiation or stimulation with GM-CSF/interleukin-4 (IL-4) (data not shown).25 Selection of HLA class I-matched donors
How do you induce U937 cells to differentiate?
U937 cells were induced to differentiate by exposing the cells (5 × 105cells/ml) to 5 ng/ml of phorbol 12-myristate 13-acetate (PMA) (Sigma, St. Louis, MO), 200 IU/ml of recombinant interferon-γ (IFN-γ) (Biomedical PBL Laboratories, New Brunswick, NJ) or 10−7m1,25-dihydroxyvitamin D3(VD3) (Roche Pharmaceuticals, Nutley, NJ), for 48 hr.
How do you differentiate THP-1 and U937 monocytes?
Differentiation of THP-1 and U937 Monocytes into Dendritic Cells THP-1 monocytes are described to differentiate into mature dendritic cells by transferring them to serum-free medium, and subsequently treating them with a mixture of IL-4, GM-CSF, TNF-α and ionomycin.
Do differentiated U937 cells generate a respiratory burst response in tuberculosis?
Differentiated U937 cells were capable of efficient phagocytosis of M. tuberculosisbut did not generate a subsequent respiratory burst response, and were permissive for intracellular growth of both bacillus Calmette–Guérin (BCG) and the virulent M. tuberculosisH37Rv strain.