What concentration of cycloheximide inhibits protein synthesis?
For all practical purpose that you indicated, a range between 5-15 microgram/ml is predicted to inhibit protein synthesis.
What is the final concentration of the ampicillin in the plates?
Ampicillin is used at a final concentration of 50 µg/ml in both LB-Ampicillin broth and LB-Ampicillin agar.
How long does it take cycloheximide to work?
Through treatment at either concentration of cycloheximide, all algae-bearing cells lost the symbiotic algae and became algae-free cells at 3–9 days after treatment. Figure 2.19.
What concentration is ampicillin?
Antibiotic Concentrations
| Antibiotic | Recommended Stock Concentration | Recommended Working Concentration |
|---|---|---|
| Ampicillin | 100 mg/mL | 100 µg/mL |
| Bleocin | 5 mg/mL | 5 µg/mL |
| Carbenicillin* | 100 mg/mL | 100 µg/mL |
| Chloramphenicol | 25 mg/mL (dissolve in EtOH) | 25 µg/mL |
How do you calculate concentration of antibiotics?
Make sure units match!
- C1V1 = C2V.
- C = concentration; V = volume.
- Example: You have a stock solution of A at 100mg/ml. You want 150 ml at 100ug/ml.
- (100ug/ml)(150ml) = (100000ug/ml)(x)
- 15000 = 100000x.
- x = 0.15ml, or 150ul.
- Remember that you have to convert mg/ml to ug/ml, which you can do by multiplying by 1000.
How would you prepare 10ml of 100mg ml ampicillin?
Directions:
- Dissolve 1 g of ampicillin into 10 ml of ddH2O.
- Filter through a 0.22 µm filter to sterilize.
- Aliquot and store at -20°C.
- Use at 1:1000 dilution in LB or LB-Agar.
How do you dilute ampicillin?
Add 1.5 ml water for injections to 500mg vial contents. Dissolve 500 mg in 10 ml water for injections. Administer by slow injection (three to four minutes). Ampicillin may also be added to infusion fluids or injected, suitably diluted, into the drip tube over a period of three to four minutes.
How long does it take for cycloheximide to work?
Through treatment at either concentration of cycloheximide, all algae-bearing cells lost the symbiotic algae and became algae-free cells at 3–9 days after treatment.
How do you dilute cycloheximide?
Preparation Instructions Cycloheximide has been reported to be soluble up to 2% (w/v) in water (20 mg/ml). dissolve slowly in water and this process may be aided by mixing or sonication. More dilute solutions (5 mg/ml) may be prepared without sonication.