Why is serum red after centrifugation?
After centrifugation a red-top tube or serum separator tube (SST). Once a whole blood specimen is hemolyzed, the hemoglobin molecules within the red blood cells are released causing the serum or plasmato have a pink to red color.
What is the salinity of blood?
between 9-10‰
Salinities. The salinity of salmonid blood plasma is between 9-10‰, about the same as other animals, including humans.
What is EDTA plasma?
EDTA plasma has an excess of a powerful chelating agent that will sequester metal ions such as calcium and magnesium. EDTA plasma will also inactivate some enzymes that require a metal ion for their activity. This includes alkaline phosphatase used as a label in some immunoassays.
How much of blood is serum?
approximately 55%
Serum is plasma without the clotting factors. Red blood cells account for approximately 45% of the whole blood, white blood cells and platelets approximately 1%, and serum or plasma accounts for the remainder, approximately 55% of the volume (Fig. 3).
Why is serum black?
Other than methaemoglobin, dark serum coloration can be caused by presence of myoglobin or methaemalbumin, which is composed of albumin bound to oxidized free heme due to intravascular haemolysis.
How much sodium is in the human body?
The human body contains many salts, of which sodium chloride (AKA common table salt) is the major one, making up around 0.4 per cent of the body’s weight at a concentration pretty well equivalent to that in seawater. So a 50kg person would contain around 200g of sodium chloride – around 40 teaspoons.
What percentage of nacl is found in blood plasma?
The sodium chloride percentage in the plasma varies between 0358 and 0.62 per cent.
What does EDTA mean in a blood test?
EDTA, short for ethylenediaminetetraacetic acid, is a fairly standard part of blood collection. It is used not only as a way to keep blood from clumping together, but also to treat extreme cases of lead poisoning in a process called “chelation therapy.”