What is a Rodac plate used for?
Rodac plates (RODAC = Replicate Organism Detection And Counting) can be used for microbiological control of all surfaces. For example for textiles (finished product inspection) or other end products and the control of folding tables, conveyor belts, trolleys, containers etc..
How do you incubate Rodac plates?
RODAC plates are prepared so that the agar surface is convex for sampling flat surfaces. Prior to sampling, the plates should be warmed to room temperature in the plastic sleeve for approximately 15-20 minutes with agar up and the lid down. Remove the quantity of plates from the sleeve that are required for testing.
When would you use a settle plate?
They can be used for passive and active air monitoring as well as glove prints. Ten plates each are single bagged. Ideal for monitoring less critical cleanroom areas, such as grade C, D and E, or non-specified environments, our single bagged long(L) incubation (I) settle plates are the ideal choice.
What temperature should you pour agar?
Water temperature should remain at around 100°C. Leave it in the water bath until the agar is completely melted. While wearing heat- protective gloves, carefully remove the hot bottle and let it cool to between 75–55°C before pouring.
What is swab method?
This document provides a method for swab sampling. Swab (or wipe) sampling can be used to detect organic and inorganic contaminants (dusts, pesticides, metals, spray drift, contaminant residues, etc.) on different surfaces.
What are settle plates and contact plates?
Settle plates are the main passive method deployed for air monitoring, contact plates and swabs are used for surface monitoring whereas primary active methods are air samplers, which sample a known volume of air over or through a collection medium.
Why are EM plates exposed for 4 hours?
According to studies, it has been noted that after a four hour period, the agar forms a skin layer on it which reduces the access of water to the microorganisms, thus reducing their growth and hindering the test by leading us to believe that there are much less viable microorganisms than there really are.