What is anti c-myc?
Anti-c-myc is a monoclonal antibody to c-myc peptide (clone 9E10). Anti-c-myc recognizes the 9E10 epitope sequence (EQKLISEEDL), derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not cross-react with other cellular proteins.
What size is myc?
The tag is approximately 1202 Daltons in atomic mass and has 10 amino acids. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
What is myc DDK tag?
A Myc-tag is a polypeptide protein tag derived from the c-myc gene product that can be fused to either the C- or N-terminus of a protein through recombinant DNA technology. It can facilitate the affinity purification of the tagged protein, as well as the isolation and detection of the tagged protein.
How does C-MYC work?
c-Myc is a multifunctional transcription factor which drives the multiple synthetic functions necessary for rapid cell division while at the same time inhibiting expression of genes with antiproliferative functions. Because of its propensity to induce apoptosis, its expression is tightly regulated.
What is the peptide sequence of the Myc-tag?
The peptide sequence of the myc-tag is (in 1- and 3-letter codes, respectively): EQKLISEEDL and Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu. The tag is approximately 1202 Daltons in atomic mass and has 10 amino acids. It can be fused to the C-terminus and the N-terminus of a protein.
What are some examples of Myc tags?
Examples are cellulite localization studies by immunofluorescence or detection by Western blotting . The peptide sequence of the myc-tag is (in 1- and 3-letter codes, respectively): EQKLISEEDL and Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu.
What are epitope tags used for in cell culture?
Epitope tags are widely used in cell culture and immunoprecipitation (IP), including protein complex immunoprecipitation (co-IP) 2 [Figure 1]. Discover commonly used epitope tags, recommended applications, and what to watch out for below. Figure 1: This diagram shows the steps for co-immunoprecipitation.
What is a c-myc tag?
The c-myc tag is derived from the c-terminal of the c-myc gene and it can be effectively recognized by antibodies. As such, it is a widely used tag for the detection of proteins in applications such as western blot, immunoprecipitation, and flow cytometry. Purified c-myc tagged proteins have been successfully crystallized 3.